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Status
Public on Apr 29, 2010
Title
NKT.4-.Lv#4
Sample type
RNA
Source name
liver
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
homogenize, ficoll, stain double sort on FACS Aria
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NKT.4-.Lv#4, CD4- iNKT cells from Liver
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
15.4174
10344622
303.0867
10344624
394.1465
10344633
894.7204
10344637
249.6596
10344653
16.0757
10344658
257.656
10344674
8.6395
10344679
46.1362
10344707
280.5248
10344713
344.7665
10344723
217.9676
10344725
37.2887
10344741
3479.9379
10344743
26.0108
10344750
92.2583
10344772
23.9936
10344789
199.741
10344797
317.1889
10344799
242.4795
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538328_EA07068_96456_MoGene_NKT.4-.LV#4.CEL.gz
4.0 Mb
(ftp) (http) CEL
Processed data included within Sample table
