GEO help: Mouse over screen elements for information.
Status
Public on Apr 29, 2010
Title
NKT.44+NK1.1+.Th#1
Sample type
RNA
Source name
thymus
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
homogenize, stain MACS, doube sort FACS Aria
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NKT.44+NK1.1+.Th#1, Developing thymic iNKT cells, stage 3
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
Jul 21, 2021
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE73864 Reanalyzed by
GSE180556
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.4391
10344622
260.6135
10344624
317.7011
10344633
789.4642
10344637
182.3678
10344653
16.3219
10344658
147.7483
10344674
11.5982
10344679
42.2257
10344707
237.4315
10344713
428.841
10344723
123.9495
10344725
39.814
10344741
3595.1737
10344743
30.8288
10344750
76.3748
10344772
25.4078
10344789
150.3138
10344797
345.9367
10344799
187.8069
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538332_EA07068_91103_MoGene_NKT.44+NK1.1+.TH#1.CEL.gz
4.3 Mb
(ftp) (http) CEL
Processed data included within Sample table
