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Sample GSM538342 Query DataSets for GSM538342
Status Public on Apr 29, 2010
Title preB.FrD.FL#3
Sample type RNA
 
Source name Fetal Liver
Organism Mus musculus
Characteristics age: 18 days
exclusion markers: CD3 Ly6c Ter119 Gr-1 CD11b
genetic background: C57BL/6J
phenotype markers: AA4.1+ IgM- CD19+ CD43- CD24+
separation method: dissection/trimming
Sex: both (pooled)
Extracted molecule total RNA
Extraction protocol Ground between 2 glass slides, ACK lysis of RBCs
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description preB.FrD.FL#3, Fr D (pre-B)
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 17.4533
10344622 260.3264
10344624 508.7595
10344633 941.8145
10344637 310.1331
10344653 12.1605
10344658 416.5483
10344674 9.7769
10344679 38.7654
10344707 456.2257
10344713 877.7696
10344723 244.0821
10344725 40.4398
10344741 3846.1206
10344743 31.046
10344750 152.0921
10344772 28.1839
10344789 388.4758
10344797 229.054
10344799 403.6984

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538342_EA07068_83960_MoGene_PREB.FRD.FL#3.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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