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Sample GSM538416 Query DataSets for GSM538416
Status Public on Apr 29, 2010
Title T.8Nve.Sp#2
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4- 8+ 25- 62Lhi 44lo
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing, FACS sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8Nve.Sp#2, Spleen Naive CD8
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date Jul 13, 2018
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE115450
Reanalyzed by GSE117080

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 21.4387
10344622 299.5201
10344624 303.8532
10344633 668.6578
10344637 217.3426
10344653 18.9801
10344658 98.3959
10344674 16.5359
10344679 58.341
10344707 206.3018
10344713 499.3426
10344723 237.711
10344725 42.7197
10344741 1828.5579
10344743 27.9137
10344750 302.6434
10344772 37.9556
10344789 111.6291
10344797 488.6699
10344799 154.8226

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538416_EA07068_52754_MoGene_T.8Nve.Sp_#2.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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