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Sample GSM5487 Query DataSets for GSM5487
Status Public on Apr 05, 2003
Title Spleen vs CD27 Treated B cell 30min (set3)
Sample type RNA
 
Channel 1
Source name Spleen was labeled with Cyanine-3 (green).
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name CD27 Treated B cells were labeled with Cyanine-5 (red)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD27 for 30min
 
Submission date Apr 04, 2003
Last update date May 27, 2005
Contact name Sangdun Choi
E-mail(s) [email protected]
Phone 626-395-8732
URL http://www.signaling-gateway.org
Organization name California Institute of Technology
Department Biology
Lab Alliance for Cellular Signaling Molecular Biology Laboratory
Street address 1200 E. California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platform ID GPL260
Series (2)
GSE375 Spleen vs CD27 Treated B cell
GSE2050 Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands

Data table header descriptions
ID_REF Feature number which correpond to the order of genes on the array
RATIO Raw Log10 Ratio
G_PROCESSED Green Processed signal, after dye normalization and background subtraction, used for computation of log ratio
R_PROCESSED Red Processed signal, after dye normalization and background subtraction, used for computation of log ratio
G_MEAN Green Channel Mean Intensity
R_MEAN Red Channel Mean Intensity
G_MEDIAN Green Channel Median Intensity
R_MEDIAN Red Channel Median Intensity
G_SD Green Channel Standard Deviation
R_SD Red Channel Standard Deviation
G_BG_MEAN Green Channel Mean Background Level
R_BG_MEAN Red Channel Mean Background Level
G_BG_MEDIAN Green Channel Median Background Level
R_BG_MEDIAN Red Channel Median Background Level
G_BG_SD Green Channel Background Standard Deviation
R_BG_SD Red Channel Background Standard Deviation
VALUE Processed Log2 Ratio after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots.

Data table
ID_REF RATIO G_PROCESSED R_PROCESSED G_MEAN R_MEAN G_MEDIAN R_MEDIAN G_SD R_SD G_BG_MEAN R_BG_MEAN G_BG_MEDIAN R_BG_MEDIAN G_BG_SD R_BG_SD VALUE
1 -0.382156 53665.3 22260.6 65517 40357.6 65517 38183 0.807726 7630.21 719.399 1250.85 720 1251 22.9639 47.1736 0
2 -0.304956 162.339 80.4392 833.958 1288.63 832 1289.5 31.7044 57.4046 711.717 1242.15 711 1241 22.2535 47.2 0
3 0.0280717 74.7302 79.72 736.038 1288.92 730 1297 34.5618 88.8175 710.415 1234.92 709 1235 22.6378 54.3268 0
4 -0.209444 89.3453 55.1604 752.08 1236.56 751 1240 23.379 49.9183 712.888 1233.49 711 1233.5 22.7999 49.4092 0
5 -0.161548 60.5649 41.7516 724.519 1223.85 718 1223 24.2048 54.9151 716.087 1246.04 717 1248 23.9837 45.9285 0
6 -0.11435 364.022 279.755 1134.68 1628 1148.5 1614 66.893 96.3274 724.733 1271.46 723 1268 23.9146 46.6996 0
7 -0.046316 65.6153 58.9779 727.107 1286.82 720.5 1295 32.348 58.211 712.296 1273.65 712 1276 23.9077 49.4622 0
8 -0.0291784 51.5513 48.2016 708.692 1254.81 713 1245 22.3924 39.7422 708.867 1262.42 707 1258.5 21.2201 47.2628 0
9 0.245409 4245.83 7470.87 6363.68 13017.3 6366 13131 178.358 837.344 720.516 1268.76 720 1268 20.901 45.8171 0.815231052
10 0.3579 710.332 1619.43 1604.92 3789.44 1443 3432 368.329 1054.33 718.093 1288.34 716 1290 20.267 48.1491 1.188918065
11 -0.0105358 53.4407 52.1599 713.231 1291.62 710 1281 18.8601 44.6982 711.273 1291.12 709.5 1284 19.5887 48.2646 0
12 0.179374 41.5759 62.8371 690.538 1280 691 1278.5 14.1428 50.5838 700.058 1255.16 698 1254 20.7995 47.3138 0
13 -0.00379525 44.5948 44.2068 690.042 1251.21 690.5 1254.5 18.4967 38.8855 697.37 1266.46 698 1264 20.4751 45.7111 0
14 0.178724 690.084 1041.42 1557.08 2815.88 1522 2768 156.319 250.122 696.576 1249.47 695.5 1252.5 20.0233 44.6546 0.593708277
15 0.199794 1911.76 3028.5 3193.5 5970.31 1980 3259 2298.75 4512.97 692.483 1252.19 692 1251 17.2555 47.6765 0
16 0.252945 1108.07 1983.87 2114.64 4351.4 1701 3873 708.138 1365.46 697.847 1274.34 696 1277.5 19.7911 54.8392 0
17 -0.340635 667.802 304.798 1517.65 1651.17 1325 1633 437.012 140.71 696.025 1269.27 695 1272 21.0906 51.4139 0
18 -0.0213839 349.345 332.561 1095.58 1685.71 1094 1681 62.0469 111.093 700.506 1244.6 699.5 1245 21.3664 46.2386 -0.071035778
19 0.518355 3913.29 12909.1 5890.11 21643 5812 21721.5 884.55 3735.53 698.774 1234.55 698 1233 18.6215 47.0964 1.721938038
20 -0.165887 5346.11 3648.81 7808.81 6935.84 7920 7051 1048.06 773.415 696.718 1252.06 698 1248 18.8609 49.6406 -0.551064686

Total number of rows: 16273

Table truncated, full table size 1996 Kbytes.




Supplementary data files not provided

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