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Sample GSM5499 Query DataSets for GSM5499
Status Public on Apr 05, 2003
Title Spleen vs CD27 Treated B cell 120min (set3)
Sample type RNA
 
Channel 1
Source name Spleen was labeled with Cyanine-3 (green).
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name CD27 Treated B cells were labeled with Cyanine-5 (red)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD27 for 120min
 
Submission date Apr 04, 2003
Last update date May 27, 2005
Contact name Sangdun Choi
E-mail(s) [email protected]
Phone 626-395-8732
URL http://www.signaling-gateway.org
Organization name California Institute of Technology
Department Biology
Lab Alliance for Cellular Signaling Molecular Biology Laboratory
Street address 1200 E. California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platform ID GPL260
Series (2)
GSE375 Spleen vs CD27 Treated B cell
GSE2050 Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands

Data table header descriptions
ID_REF Feature number which correpond to the order of genes on the array
RATIO Raw Log10 Ratio
G_PROCESSED Green Processed signal, after dye normalization and background subtraction, used for computation of log ratio
R_PROCESSED Red Processed signal, after dye normalization and background subtraction, used for computation of log ratio
G_MEAN Green Channel Mean Intensity
R_MEAN Red Channel Mean Intensity
G_MEDIAN Green Channel Median Intensity
R_MEDIAN Red Channel Median Intensity
G_SD Green Channel Standard Deviation
R_SD Red Channel Standard Deviation
G_BG_MEAN Green Channel Mean Background Level
R_BG_MEAN Red Channel Mean Background Level
G_BG_MEDIAN Green Channel Median Background Level
R_BG_MEDIAN Red Channel Median Background Level
G_BG_SD Green Channel Background Standard Deviation
R_BG_SD Red Channel Background Standard Deviation
VALUE Processed Log2 Ratio after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots.

Data table
ID_REF RATIO G_PROCESSED R_PROCESSED G_MEAN R_MEAN G_MEDIAN R_MEDIAN G_SD R_SD G_BG_MEAN R_BG_MEAN G_BG_MEDIAN R_BG_MEDIAN G_BG_SD R_BG_SD VALUE
1 -0.182214 36059.9 23703.4 65516.8 44281.8 65517 40723.5 0.645497 12423.8 1033.98 2254.69 1031 2247 44.3163 125.395 0
2 -0.448151 131.457 46.8419 1123.2 2122.16 1125 2098 62.7887 144.065 1045.68 2255.04 1047 2250.5 44.1854 108.768 0
3 0.15939 79.0601 114.116 1051.85 2291.22 1052 2292 37.6979 101.866 1038.29 2232.11 1035.5 2227 42.0228 97.3202 0
4 -0.233462 72.341 42.2593 1016.04 1972.35 1025.5 2012.5 27.236 136.471 1035.34 2215.94 1033 2216.5 35.8085 116.422 0
5 -0.308714 97.7859 48.0355 1081.64 2099.08 1055 2162 78.944 177.969 1055.84 2265.24 1055.5 2263 39.3222 116.273 0
6 -0.185694 373.113 243.302 1648.3 2499.74 1646 2515 120.574 175.554 1060.89 2268 1059 2265.5 40.4626 116.257 0
7 0.157671 69.2909 99.6203 1051.59 2333.37 1052 2350 35.6201 130.466 1054.94 2300.19 1051 2291 39.019 107.484 0
8 0.0671381 73.7272 86.053 1060.52 2305.61 1050 2319 42.7529 97.1988 1058.15 2301.3 1053 2288 42.099 123.083 0
9 0.17087 4107.81 6088.09 8479.92 12657.9 8451 12599 356.027 719.889 1052.68 2251.9 1047 2254 44.9962 116.973 0.567617854
10 0.69841 493.357 2463.6 1863.86 6436.64 1885.5 6440 249.934 1443.26 1065.89 2321.44 1060 2316 43.8523 97.0326 2.320067801
11 -0.162267 68.5205 47.1578 1074.16 2291.96 1073 2287 43.6384 58.2241 1083.11 2374.92 1081 2366 34.4608 109.52 0
12 0.10589 78.2464 99.8514 1096.75 2428.79 1087 2425.5 38.916 70.4174 1086.04 2397.38 1082 2396.5 36.7566 125.453 0
13 0.0227587 80.8242 85.1727 1120.64 2454.04 1119 2454 40.8625 93.4217 1107.42 2452.84 1106 2452 41.6227 115.715 0
14 0.287056 641.543 1242.46 2204.46 4450.29 2218 4374.5 197.432 609.762 1135.44 2468.66 1129 2460 43.7911 125.272 0.953579391
15 0.202414 1985.24 3163.92 4667.52 7807.76 3104 5392 3191.67 4610.84 1126.54 2497.2 1124 2492.5 46.0666 114.537 0
16 0.287407 901.044 1746.44 2659.27 5336.04 2338 4542.5 813.994 1457.67 1115.88 2474.98 1111.5 2474 45.3801 136.805 0
17 -0.267109 619.052 334.671 2105.79 2613.21 1714 2511.5 898.093 309.712 1062.78 2224.22 1062 2209.5 41.1065 144.278 0
18 0.186047 306.617 470.59 1494.33 2686 1507 2701 84.7957 138.412 1028.07 2065.21 1029 2054 31.6691 102.651 0.618034756
19 0.376314 4047.74 9627.78 8326.44 18575.2 8745 19103 1080.66 2125.47 1011.85 2004.26 1012.5 2010 35.5728 101.215 1.250088049
20 0.0552736 4039.86 4588.17 8318.04 9778.74 8415 9645 860.464 899.8 1011.16 1987.21 1014 1994.5 31.2482 81.1227 0.183614925

Total number of rows: 16273

Table truncated, full table size 2000 Kbytes.




Supplementary data files not provided

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