|
| Status |
Public on May 13, 2011 |
| Title |
AP1510 979-CLA-28 |
| Sample type |
RNA |
| |
|
| Source name |
MCF10A-HER2-FKBP-HA cells
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: normal breast cell line with stably transfected full-length chimeric human HER2 receptor with FKBP and HA tags
cell line: MCF10A
treatment: AP1510
|
| Treatment protocol |
Cells were seeded in 100 mm dishes in triplicate. 24 hours before ligand treatment, media was changed to DMEM/F12 + 100ng/ml cholera toxin + 500ng/ml hydrocortisone + 10microgram/ml insulin. RNA was harvested 48 hours after addition of ligand.
|
| Growth protocol |
Cells are maintained in DMEM/F12 + 5% horse serum + 20ng/ml EGF + 100ng/ml cholera toxin + 500ng/ml hydrocortisone + 10microgram/ml insulin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using Ambion ToTALLY RNA kit
|
| Label |
biotin
|
| Label protocol |
GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix)
|
| |
|
| Hybridization protocol |
5.5 ug biotinylated cRNA was fragmented and hybridizied to an Affymetrix Genechip Human Gene 1.0 ST array
|
| Scan protocol |
Hybridized cRNA was detected using streptavidin coupled to phycoerythrin. Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix)
|
| Description |
Gene expression data from cells treated with AP1510 for 48 hours
|
| Data processing |
Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
RMA
|
| |
|
| Submission date |
Jun 10, 2010 |
| Last update date |
May 13, 2011 |
| Contact name |
Ritwik Ghosh |
| Organization name |
Vanderbilt University
|
| Street address |
2220 Pierce Avenue, 777 PRB
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE22288 |
Controlled ErbB receptor dimerization |
|
