|
| Status |
Public on Jun 15, 2010 |
| Title |
cone at T220, biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse retinal cones, dissected after 220 days
|
| Organism |
Mus musculus |
| Characteristics |
strain: C3H
mouse model: f-RD
tissue: retina
cell type: RD cone photoreceptors
genotype/variation: C3H/HeNCrl (Pde6brd1)
age: 220 days
treatment protocol: mCAR-EGFP
|
| Treatment protocol |
AAVs were subretinally administrated in P21-28 old mice. Mice were kept until indicated ages and used for gene array experiments.
|
| Growth protocol |
All animal experiments and procedures were approved by the Swiss Veterinary Office. The animals were maintained under a 12-hour light-dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the PicoPure RNA Isolation kit manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2005, Rev2 and GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual, Rev4, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 7000 7G (Affymetrix).
|
| Description |
Gene expression data of 500 FACSed RD cones at 220 days
|
| Data processing |
The CEL files were aquired using the GeneChip Command Console (Affymetrix). Quality control and gene expression value estimates were obtained after RMA and quantile normalization in R (Bioconductor 'Affy' package).
|
| |
|
| Submission date |
Jun 14, 2010 |
| Last update date |
Jun 15, 2010 |
| Contact name |
Erik Cabuy |
| E-mail(s) |
[email protected]
|
| Phone |
+41 616976895
|
| URL |
http://www.fmi.ch
|
| Organization name |
Friedrich Miescher Institute
|
| Department |
Single Cell Genomics
|
| Lab |
WRO-1066.1.58
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE22338 |
Expression data from cones in degenerated retinas from C3H/HeNCrl (Pde6brd1) mice |
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