|
| Status |
Public on Jan 24, 2011 |
| Title |
Adipose-Beef 2 |
| Sample type |
RNA |
| |
|
| Source name |
Ldlr-/- beef
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Ldlr-/-
tissue: Adipose
diet: Beef-fed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy mini kit or the RNeasy Lipid tissue mini kit (QIAGEN), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix)
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix) by A GeneChip Fluidics Station FS-400 and GeneChip Scanner 3000 7G
|
| Description |
Adipose-Beef 2
|
| Data processing |
Probe Logarithmic Intensity Error (PLIER)
|
| |
|
| Submission date |
Jun 23, 2010 |
| Last update date |
Jan 24, 2011 |
| Contact name |
Intawat Nookaew |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Department of Biomedical Informatics
|
| Street address |
4301 W Markham St
|
| City |
Little Rock |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE22532 |
Identifying Molecular Effects of Diet through Systems Biology: Influence of Herring Diet on Sterol Metabolism and Protein Turnover in Mice |
|
