|
| Status |
Public on Oct 07, 2021 |
| Title |
OX.CARPO_Syrah_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
OX.CARPO leaves of Vitis vinifera cv. Syrah_rep2
|
| Organism |
Vitis vinifera |
| Characteristics |
cultivar: Syrah
genotype: CARPO overexpression
tissue: Fully expanded leaves
|
| Growth protocol |
Grapevine plants (Vitis vinifera cv. Syrah, BVRC12 clone) were grown at the University of Verona greenhouse, starting from calli developed from anthers collected during the 2009 seasons in Coombe Vineyard (University of Adelaide, Urrbrae, South Australia). For the microarray analysis performed on grapevine, three biological replicates of fully expanded leaves were collected from control and transgenic plants.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from ~100 mg of the ground grapevine leaves tissue using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich).
|
| Label |
Cy3
|
| Label protocol |
For microarray analysis on Syrah leaves stably overexpressing VviCARPO, the cDNA synthesis and labeling reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
|
| |
|
| Hybridization protocol |
For microarray analysis on Syrah leaves stably overexpressing VviCARPO, the hybridization and washing reactions have been conducted according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
|
| Scan protocol |
Scanning and Feature Extraction were performed by using an Agilent Scanner following the settings and parameters indicated in the instruction manual.
|
| Data processing |
A data-matrix was prepared selecting from each single sub-array outcome file the gProcessedSignalvalues, which are the raw fluorescence intensities of each probe. The data were normalized on the 75th percentile and a correlation analysis was then performed to assess the consistency of the biological quadruplicates.
|
| |
|
| Submission date |
Oct 06, 2021 |
| Last update date |
Oct 07, 2021 |
| Contact name |
Sara Zenoni |
| Organization name |
Università degli Studi di Verona
|
| Street address |
Strada Le Grazie 15
|
| City |
Verona |
| ZIP/Postal code |
37134 |
| Country |
Italy |
| |
|
| Platform ID |
GPL22427 |
| Series (2) |
| GSE185448 |
Initiation of organ maturation and fruit ripening in grapevine is controlled by the CARPO-NAC transcription factor [Syrah] |
| GSE185449 |
Initiation of organ maturation and fruit ripening in grapevine is controlled by the CARPO-NAC transcription factor |
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