|
| Status |
Public on Mar 14, 2023 |
| Title |
BEC_AsthmaHDM_DAY14-DJ139 |
| Sample type |
RNA |
| |
|
| Source name |
BEC
|
| Organism |
Homo sapiens |
| Characteristics |
time: DAY14
group: AsthmaHDM
donor: DJ139
|
| Treatment protocol |
Control individuals and patients with asthma were inoculated intranassaly with 100 TCID50 of rhinovirus A16. Bronchial brushings from around two weeks before (day -14) and four days after infection (day 4) were collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the use of RNAeasy Mini kit (Qiagen) according to manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Samples were processed for HuGene 1.0 array following the manufacturers instructions (Affymetrix).
|
| Scan protocol |
The arrays were scanned by the high-resolution scanner of GeneChip Scanner (Affymetrix)
|
| Data processing |
Raw data were normalized using justRMA() from affy R package.
|
| |
|
| Submission date |
Oct 11, 2021 |
| Last update date |
Mar 14, 2023 |
| Contact name |
Ge Tan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Zurich
|
| Department |
Functional Genomics Center Zurich
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE185658 |
Rhinovirus-induced epithelial RIG-I inflammasome suppresses antiviral immunity and promotes inflammation in asthma and COVID-19 |
|
