|
| Status |
Public on Sep 15, 2022 |
| Title |
hPSCs at Day 0 of differentiation (D0_A05)_gene |
| Sample type |
RNA |
| |
|
| Source name |
hPSCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hPSCs
age: d0
cell lineage: Coriell Cell Repositories (AICS-0037-172)
|
| Biomaterial provider |
Coriell Cell Repositories (AICS-0037-172)
https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AICS-0037-172&PgId=166
|
| Treatment protocol |
Cardiac cell differentiation protocol followed Burridge et al., 2014 specifications. No treatment was applied after cell differentiation.
|
| Growth protocol |
Prior to cardiac differentiation hPSC cells were maintained in E8:mTeSR [(1:1), Stemcell Technologies, #85850] with daily change. Established hPSC-CM were cultivated with RPMI-1640 supplemented with B27 and insulin until the end of protocol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We used the RNeasy Micro Kit (Qiagen) for total RNA isolation
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using RNA samples for whole transcriptome expression analysis with GeneChipTM Whole Transcript (WT) Expression Arrays (appliedbiosystems)
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChipTM WT PLUS Reagent Kit (Affymetrix) and scanned at GeneTitan ™ Microarray System
|
| Scan protocol |
The entire protocol for processing, running and scanning the samples followed the manufacturer's instruction [GeneChip ™ WT PLUS Reagent Kit Manual] at GeneTitan ™ Microarray System
|
| Data processing |
The .CEL files were analyzed using the Transcriptome Analysis Console software (TAC v4.0) from AppliedBiosystems
|
| |
|
| Submission date |
Nov 12, 2021 |
| Last update date |
Sep 15, 2022 |
| Contact name |
Jose Eduardo Krieger |
| E-mail(s) |
[email protected]
|
| Phone |
+551126615573
|
| Organization name |
University of Sao Paulo
|
| Department |
Medical School
|
| Lab |
Laboratory of Genetics and Molecular Cardiology (LGCM)
|
| Street address |
44 Av. Dr. Enéas de Carvalho Aguiar
|
| City |
Sao Paulo |
| State/province |
Sao Paulo |
| ZIP/Postal code |
05403-000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL24324 |
| Series (2) |
| GSE188725 |
Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation [gene] |
| GSE188749 |
Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation |
|
