|
| Status |
Public on Nov 01, 2010 |
| Title |
atrium_SlnKO_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse SlnKO atrium
|
| Organism |
Mus musculus |
| Characteristics |
genotype: sln-/-
tissue: heart atrium
gender: male
age: 4-5 months
|
| Treatment protocol |
No treatment prior to experiment was applied.
|
| Growth protocol |
No growth protocol. Atrial tissues were taken from 4-5 months old SLN knockout and C57/BL6 control mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA as described by Affymetrix.
|
| |
|
| Hybridization protocol |
RNA samples were hybridizaed with Affymetrix expression arrays offering whole-transcript coverage. After hybridization, non-specific binding was washed off according to the manufacturer's protocol.
|
| Scan protocol |
Affymetrix arrays were scanned with the Affymetrix GeneArray scanner.
|
| Description |
Gene expression data from Sln knockout
|
| Data processing |
In the Expression Console software, hybridization intensity data were normalized using the Robust Multichip Average (RMA) method.
|
| |
|
| Submission date |
Aug 03, 2010 |
| Last update date |
Aug 04, 2010 |
| Contact name |
Gopal J Babu |
| E-mail(s) |
[email protected]
|
| Phone |
973-972-5376
|
| Fax |
973-972-7489
|
| Organization name |
University of Medicine & Dentistry of New Jersey-NJMS
|
| Department |
CELL BIOLOGY AND MOLECULAR MED
|
| Street address |
185 South Orange Ave
|
| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07101 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE23403 |
Loss of sarcolipin results in atrial remodeling |
|
