|
| Status |
Public on Sep 27, 2010 |
| Title |
JIA PBMC Pre treatment A235_L221 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMC
responder: ACR-Ped70
treatment: Methotrexate pre-treatment
subject: A235_L221
|
| Treatment protocol |
This was a non-interventional study. Juvenile idiopathic arthritis patients were treated with methotrexate and samples collected prior to treatment and after 6 months on therapy.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 3x10e6 PBMC using TRIzol reagent (Invitrogen, UK)
|
| Label |
biotin
|
| Label protocol |
RNA was coverted to cDNA (Roche) using an oligod(T) primer and converted to cRNA using In Vitro Triscription labelling kit (Affymetrix
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix GeneChip Human U133 2.0 array. GeneChips were washed and stained on the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000.
|
| Description |
JIA PBMC Pre treatment
|
| Data processing |
RMA
|
| |
|
| Submission date |
Aug 18, 2010 |
| Last update date |
Aug 19, 2010 |
| Contact name |
Lucy R Wedderburn |
| Organization name |
Institute of Child Health, UCL
|
| Department |
Rheumatology
|
| Street address |
30 Guilford Street
|
| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23687 |
Expression data from SPARKS CHARMS JIA cohort |
|
