|
| Status |
Public on Feb 01, 2022 |
| Title |
ahr-1(ju145)_100µM curcumin_2 [ahr C2] |
| Sample type |
RNA |
| |
|
| Source name |
3-days old C. elegans treated with 100µM curcumin from eggs until day 3
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: CZ2485
genotype: ahr-1(ju145)
treatment: 100uM curcumin from eggs until day 3
|
| Growth protocol |
nematodes were grown on NGM plates supplemented with either 0.1% DMSO or 100 µM curcumin from eggs to day 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
after disruption of the worms using a Tissue Lyser the total RNA was extracted using the Rneasy kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
Samples were prepared and hybridized to C. elegans Gene 1.0 ST Microarray according to Affymetrix specifications.
|
| |
|
| Hybridization protocol |
Samples were prepared and hybridized to C. elegans Gene 1.0 ST Microarray according to Affymetrix specifications.
|
| Scan protocol |
The tiling arrays were scanned using an Affymetrix GeneChip Scanner 3000 7G according to manufacturer's instruction.
|
| Data processing |
RMA normalization; normalized also per gene to the median
|
| |
|
| Submission date |
Jan 31, 2022 |
| Last update date |
Feb 01, 2022 |
| Contact name |
Vanessa Brinkmann |
| E-mail(s) |
[email protected]
|
| Organization name |
IUF- Leibniz Research Institute for Environmental Medicine
|
| Lab |
Ventura Lab
|
| Street address |
Auf'm Hennekamp 50
|
| City |
Düsseldorf |
| State/province |
NRW |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL19230 |
| Series (1) |
| GSE195769 |
Aryl hydrocarbon receptor-dependent and -independent pathways mediate curcumin anti-aging effects |
|
