|
| Status |
Public on May 15, 2023 |
| Title |
4_xlg_2h rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Seedlings
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
tissue: Seedlings
age: 7-day-old
genotype: xlg1,4
|
| Treatment protocol |
1/2 Ms + 100 mM NaCl treatment for 0, 2 and 24 hr, 1/2 MS + 0.3 ug/ mL tunicamycin for 4 hr
|
| Growth protocol |
1: 7-day-old arabidopsis seedlings liquid grown in 1/2 MS, continuous light; 2-3: 7-day-old marchantia gemma liquid grown in 1/2 B5, continuous light; 4: 7-day-old NB seedlings grwon on 1/2 MS plates, 16/8 Light/Dark cycle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
1-4: Plant samples were frozen in liquid nitrogen, and followed by RNA extraction using Rneasy Plant Mini Kit (Qiagen)
1-4: RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
4_raw_reads_counts
|
| Data processing |
1-4: STAR for genome mapping
Assembly: Arabidopisis: TAIR10; Marantia v3.1, v5.1; Rice: IRGSP 1.0
Supplementary files format and content: 1-4: raw counts
|
| |
|
| Submission date |
Mar 28, 2022 |
| Last update date |
May 15, 2023 |
| Contact name |
Daisuke Urano |
| Organization name |
Temasek Life Science Laboratory
|
| Street address |
1 Research Link
|
| City |
Sinagpore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL27860 |
| Series (1) |
| GSE199590 |
G-protein mutants RNA-seq analysis of salt resonse in Arabidopsis and Marchantia |
|
| Relations |
| BioSample |
SAMN27023072 |
| SRA |
SRX14637692 |
