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Sample GSM605802 Query DataSets for GSM605802
Status Public on Oct 08, 2010
Title Tgd.vg5+.act.IEL#1
Sample type RNA
 
Source name small intestine
Organism Mus musculus
Characteristics age: 5 weeks
genetic background: C57BL/6J
phenotype markers: CD8a+ CD8b- TCRd+ Vg5+ CD44hi
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Mincing / Shaking / Percoll gradient, Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Tgd.vg5+.act.IEL#1, small intestine TCRd+ Vg5+ CD44+ CD8aa+
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Oct 07, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.7519
10344622 261.1419
10344624 466.855
10344633 686.0358
10344637 313.2695
10344653 13.2783
10344658 268.5866
10344674 11.4067
10344679 47.091
10344707 446.4904
10344713 375.0489
10344723 300.7864
10344725 40.1205
10344741 3553.7229
10344743 26.8668
10344750 116.0529
10344772 23.3729
10344789 204.7261
10344797 235.0366
10344799 233.7162

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM605802_EA07068_56609_MoGene_Tgd.vg5+.act.IEL_#1.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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