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Sample GSM605814 Query DataSets for GSM605814
Status Public on Oct 08, 2010
Title NK.49H+.Sp#4
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: dump, dead
genetic background: C57BL/6J
phenotype markers: NK1.1+ Ly49H+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide, Ack lysis
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description NK.49H+.Sp#4, Splenic NK cells expressing Ly49H receptor
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Oct 07, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.0339
10344622 417.194
10344624 457.0969
10344633 656.0553
10344637 204.6195
10344653 17.8305
10344658 190.0722
10344674 11.8626
10344679 47.8036
10344707 321.5607
10344713 337.6473
10344723 175.9097
10344725 48.3549
10344741 1876.8217
10344743 34.0713
10344750 130.3503
10344772 25.0762
10344789 129.9675
10344797 369.7023
10344799 183.166

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM605814_EA07068_108027_MoGene_NK.49H+.SP#1.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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