GEO help: Mouse over screen elements for information.
Status
Public on Oct 08, 2010
Title
NK.49H+.Sp#4
Sample type
RNA
Source name
spleen
Organism
Mus musculus
Characteristics
age: 6 weeks exclusion markers: dump, dead genetic background: C57BL/6J phenotype markers: NK1.1+ Ly49H+ separation method: dissection/trimming Sex: male
Extracted molecule
total RNA
Extraction protocol
Glass slide, Ack lysis
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NK.49H+.Sp#4, Splenic NK cells expressing Ly49H receptor
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246
Series (1)
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
16.0339
10344622
417.194
10344624
457.0969
10344633
656.0553
10344637
204.6195
10344653
17.8305
10344658
190.0722
10344674
11.8626
10344679
47.8036
10344707
321.5607
10344713
337.6473
10344723
175.9097
10344725
48.3549
10344741
1876.8217
10344743
34.0713
10344750
130.3503
10344772
25.0762
10344789
129.9675
10344797
369.7023
10344799
183.166
Total number of rows: 24922 Table truncated, full table size 422 Kbytes .Supplementary file
Size
Download
File type/resource
GSM605814_EA07068_108027_MoGene_NK.49H+.SP#1.CEL.gz
4.2 Mb
(ftp) (http)
CEL
Processed data included within Sample table