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Status
Public on Oct 08, 2010
Title
NK.49H-.Sp#4
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Glass slides, Ack lysis
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
NK.49H-.Sp#4, Splenic NK cells lacking Ly49H receptor
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.0197
10344622
357.6082
10344624
501.6519
10344633
618.8648
10344637
177.8551
10344653
15.3111
10344658
257.6643
10344674
11.4173
10344679
38.168
10344707
244.7391
10344713
322.444
10344723
162.5114
10344725
45.2698
10344741
1763.3841
10344743
21.6522
10344750
139.2369
10344772
27.9822
10344789
130.948
10344797
300.8325
10344799
152.8749
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605817_EA07068_108030_MoGene_NK.49H-.SP#1.CEL.gz
4.0 Mb
(ftp) (http) CEL
Processed data included within Sample table
