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Status
Public on Oct 08, 2010
Title
DC.4+.Sp.ST#4
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Collagenase, dispase, DNAse digestion, CD3e, CD19, GR1, NK1.1 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.4+.Sp#4, CD4 CDs
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
Jul 13, 2018
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE68399 Reanalyzed by
GSE74316 Reanalyzed by
GSE115450 Reanalyzed by
GSE117080
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
18.1843
10344622
186.3577
10344624
406.1621
10344633
1008.8884
10344637
284.3467
10344653
16.6454
10344658
242.4493
10344674
9.0826
10344679
46.4188
10344707
220.9619
10344713
235.8128
10344723
222.562
10344725
43.272
10344741
3586.7635
10344743
23.8243
10344750
1196.6233
10344772
35.8278
10344789
234.4233
10344797
275.0518
10344799
279.978
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605826_EA07068_90310_MoGene_DC2.SP#4.CEL.gz
4.6 Mb
(ftp) (http) CEL
Processed data included within Sample table
