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Status
Public on Oct 08, 2010
Title
DC.8-.Th#1
Sample type
RNA
Source name
thymus
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
gentle teasing using forceps, Liberase DL + DNAse I digestion, red blood cell lysis, depletion of NK1.1, GR1, CD19, CD3e, Ter119 positive cells using MACS system
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8-.Th#1, thymus CD8- DCs
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
13.595
10344622
231.0154
10344624
692.0088
10344633
875.9148
10344637
386.0081
10344653
15.8441
10344658
161.0981
10344674
8.0258
10344679
34.6785
10344707
131.5486
10344713
640.6446
10344723
302.7004
10344725
43.5225
10344741
3474.3433
10344743
19.8397
10344750
951.2826
10344772
69.6252
10344789
205.5553
10344797
184.347
10344799
220.8739
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605831_EA07068_108115_MoGene_DC.8-.TH#1.CEL.gz
4.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
