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Status
Public on Oct 08, 2010
Title
DC.8-4-11b-.MLN#1
Sample type
RNA
Source name
mesenteric lymph node
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Blendzyme liberase III + DNase digestion, CD3, CD19, NK1.1, GR1, Ter119 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8-4-11b-.MLN#1, CD11c+, CD8a-, CD4-, CD11b-
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.5536
10344622
223.8435
10344624
340.1797
10344633
958.4758
10344637
306.2259
10344653
16.6597
10344658
280.9838
10344674
9.1658
10344679
34.5811
10344707
262.2434
10344713
142.2411
10344723
59.775
10344725
34.3315
10344741
2691.3343
10344743
24.8301
10344750
755.1724
10344772
40.0574
10344789
248.6529
10344797
321.6644
10344799
299.4015
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605834_EA07068_91088_MoGene_DC4.MLN#1.CEL.gz
4.5 Mb
(ftp) (http) CEL
Processed data included within Sample table
