GEO help: Mouse over screen elements for information.
Status
Public on Oct 08, 2010
Title
DC.8-4-11b-.MLN#6
Sample type
RNA
Source name
mesenteric LNs
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
digestion using Liberase III + DNase I, CD3, CD19, GR1, NK1.1, Ter-119 using MACS beads
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8-4-11b-.MLN#6, triple negative: CD11c+, CD11b-, CD4-, CD8-
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
23.8801
10344622
239.7004
10344624
321.8305
10344633
726.6157
10344637
306.5214
10344653
20.428
10344658
237.7348
10344674
13.2724
10344679
56.209
10344707
200.9528
10344713
139.3031
10344723
35.4923
10344725
52.345
10344741
1770.5839
10344743
30.2924
10344750
626.342
10344772
27.5003
10344789
135.0047
10344797
282.4724
10344799
229.2417
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605836_EA07068_96401_MoGene_DC.8-4-11B-.MLN#[email protected]
3.6 Mb
(ftp) (http) CEL
Processed data included within Sample table
