GEO help: Mouse over screen elements for information.
Status
Public on Oct 08, 2010
Title
DC.pDC.8+.Sp#1
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
gentle teasing with tweezers, Blendzyme Liberase III and DNAse I digestion, depletion of CD3e, CD19, NK1.1 and TER119 positive cells using MACS system
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.pDC.8+.Sp#1, spleen CD8+ plasmacytoid dentritic cells
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
Jun 07, 2018
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE74316 Reanalyzed by
GSE115450
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.5915
10344622
225.2803
10344624
424.644
10344633
793.6277
10344637
327.0092
10344653
17.4835
10344658
243.4289
10344674
15.7418
10344679
36.5063
10344707
307.0675
10344713
232.5177
10344723
137.9182
10344725
38.4578
10344741
2430.9571
10344743
24.341
10344750
743.5752
10344772
39.8973
10344789
177.0837
10344797
240.8525
10344799
228.4208
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605840_EA07068_105309_MoGene_DC.PDC.8+.SP#1.CEL.gz
4.3 Mb
(ftp) (http) CEL
Processed data included within Sample table
