RNA was purified from 2 biological replicates in each group using Trizol/Phenol-Chloroform extraction method (Invitrogen). Labeled cRNA was produced and hybridized according to manufacturer's instructions (www.affymetrix.com), at the Center for Genomic Technologies, the Hebrew University. Gene intensities were extracted from CEL files using Affymetrix Expression Console 1.1, using the RMA Sketch method. Raw data was normalized using the median intensities (Irizarry et al., 2003).
Label
biotin
Label protocol
RNA was purified from 2 biological replicates in each group using Trizol/Phenol-Chloroform extraction method (Invitrogen). Labeled cRNA was produced and hybridized according to manufacturer's instructions (www.affymetrix.com), at the Center for Genomic Technologies, the Hebrew University. Gene intensities were extracted from CEL files using Affymetrix Expression Console 1.1, using the RMA Sketch method. Raw data was normalized using the median intensities (Irizarry et al., 2003).
Hybridization protocol
RNA was purified from 2 biological replicates in each group using Trizol/Phenol-Chloroform extraction method (Invitrogen). Labeled cRNA was produced and hybridized according to manufacturer's instructions (www.affymetrix.com), at the Center for Genomic Technologies, the Hebrew University. Gene intensities were extracted from CEL files using Affymetrix Expression Console 1.1, using the RMA Sketch method. Raw data was normalized using the median intensities (Irizarry et al., 2003).
Scan protocol
RNA was purified from 2 biological replicates in each group using Trizol/Phenol-Chloroform extraction method (Invitrogen). Labeled cRNA was produced and hybridized according to manufacturer's instructions (www.affymetrix.com), at the Center for Genomic Technologies, the Hebrew University. Gene intensities were extracted from CEL files using Affymetrix Expression Console 1.1, using the RMA Sketch method. Raw data was normalized using the median intensities (Irizarry et al., 2003).
Data processing
RNA was purified from 2 biological replicates in each group using Trizol/Phenol-Chloroform extraction method (Invitrogen). Labeled cRNA was produced and hybridized according to manufacturer's instructions (www.affymetrix.com), at the Center for Genomic Technologies, the Hebrew University. Gene intensities were extracted from CEL files using Affymetrix Expression Console 1.1, using the RMA Sketch method. Raw data was normalized using the median intensities (Irizarry et al., 2003).
