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| Status |
Public on Oct 23, 2010 |
| Title |
MPTP-treated mouse 2 cervical spinal cord regions |
| Sample type |
RNA |
| |
|
| Source name |
MPTP-treated C57BL/6 mouse cervical spinal cord regions
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: C57BL/6
tissue: Cervical spinal cord tissues
developmental stage: Adult
treatment: MPTP-treated
|
| Treatment protocol |
While mice in the control group (n=9) were intraperitoneally injected with saline 0.9% (100 µl) once daily for four weeks, the mice in the MPTP group (n=9) were intraperitoneally injected with MPTP-HCl (20 mg/kg of free base) dissolved in saline 0.9% (100 µl) at twenty-four hr intervals for four weeks to produce the sustained chronic model of parkinsonism. Acupuncture was performed by hand two hr after the first MPTP injection and then at forty-eight hr intervals (fourteen total sessions). The acupuncture procedure (acupoint GB34 (Yanglingquan) and acupoint LR3 (Taichong) for acupoints; both sides of the hips for non-acupoints) was performed as previously reported. Mice in the acupoints group were immobilized by hand two hr after MPTP administration. Acupuncture needles were inserted bilaterally to depths of 1 mm at acupoint LR3 and 3 mm at acupoint GB34, and then turned at a rate of two spins per sec for fifteen sec as reported in a previous study. For the non-acupoints group, the needles were inserted to depths of 3 mm at both sides of the hips rather than at the acupoints GB34 and LR3 for the acupoints group, and then the same procedures were performed as with the acupoints.
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| Growth protocol |
Six-week old male inbred C57BL/6 mice (20-22 g) were grown up.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA in the cervical spinal cord tissues of each group (n=2 per each group) was extracted using an RNeasy Plus Mini kit (QIAGEN, USA) according to the manufacturer’s instruction.
|
| Label |
biotin
|
| Label protocol |
Fragmented and biotinylated cDNA were prepared according to the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labeling Assay manual from 300 ng total RNA (GeneChip Whole Transcript (WT) Sense Target Labeling Assay manual, Affymetrix).
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| |
|
| Hybridization protocol |
Following labeling, each cDNA was hybridized for 17 hr at 45℃ at 60 rpm on Affymetrix MouseGene 1.0 ST Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450_0007.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix)
|
| Description |
Gene expression data from cervical spinal cord regions of MPTP-intoxicated mouse
|
| Data processing |
The quality control was estimated by confirming the order of the signal intensities of the Poly-A controls and Hybridization controls using Expression Console Software (Affymetrix, USA). The microarray data was analyzed using a GenPlex V3.0 (ISTECH, Korea). The data were analyzed with Robust Multichip Analysis (RMA) quantification method as a probe set summarization algorithm for log transformation with base 2 (log2) and Quantile normalization method as a normalization method in the Preprocessing module of GenPlex V3.0. The mean signal intensities of all genes (35,557 probes (28,853 genes) in a platform) were obtained from 2 chips of each experimental group.
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| |
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| Submission date |
Oct 20, 2010 |
| Last update date |
Oct 22, 2010 |
| Contact name |
Sabina Lim |
| E-mail(s) |
[email protected]
|
| Phone |
82-2-961-0324
|
| Organization name |
East-West Medical Research Institute, Kyung Hee University
|
| Lab |
Research Group of Pain and Neuroscience
|
| Street address |
#1 Hoegi-dong Dongdaemoon-gu
|
| City |
Seoul |
| ZIP/Postal code |
130-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE24830 |
Gene expression data from cervical spinal cord regions of MPTP-intoxicated mouse by acupuncture |
| GSE24838 |
Gene expression data from brain striatal and spinal cord regions of MPTP-intoxicated mouse following acupuncture |
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