|
| Status |
Public on Apr 11, 2011 |
| Title |
BPN, 26 weeks old, 2 |
| Sample type |
RNA |
| |
|
| Source name |
Male BPN/3J hypothalamus, 26 weeks old
|
| Organism |
Mus musculus |
| Characteristics |
strain: BPN/3J
gender: male
age: 26 weeks
tissue: hypothalamus
blood pressure: normotensive
|
| Treatment protocol |
Standard animal house treatment.
|
| Growth protocol |
Hypothalamus was removed from BPH/2J and BPN/3J strains of mice at peak blood pressure. Tissues were frozen and then RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNeasy kit (Qiagen) was used for extraction of total RNA according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was prepared for hybridisation according to the standard Affymetrix protocol (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual, version 4, Affymetrix).
|
| |
|
| Hybridization protocol |
After fragmentation, ssDNA was hybridized for 17 hr at 45°C to GeneChip Mouse Gene 1.0 ST Arrays. Chips were washed and stained in the Affymetrix GeneChip® Fluidics Stations 450. For all of these steps, the GeneChip Hybridization, Wash, and Stain Kit (Affymetrix) was used according to the manufacturer’s recommendations (GeneChip® Whole Transcript (WT) Sense Target Labelling Assay Manual, version 4, Affymetrix).
|
| Scan protocol |
The chips were scanned using the GeneChip® Scanner 3000 7G 4C (Affymetrix) according to the manufacturer’s recommendations (GeneChip® Whole Transcript (WT) Sense Target Labelling Assay Manual, version 4, Affymetrix).
|
| Description |
BPN6
Gene expression from adult normotensive BPN/3J mouse.
|
| Data processing |
The samples were individually normalized using robust-multi-array analysis (RMA) via the package 'Affy' in the R application (version 2.11.1). Differentially expressed genes for each age were identified using the moderated two-sample t-test in the Limma package.
|
| |
|
| Submission date |
Nov 04, 2010 |
| Last update date |
Apr 11, 2011 |
| Contact name |
Prof.Brian J Morris |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Sydney
|
| Department |
Physiology
|
| Lab |
Basic & Clinical Genomics Lab
|
| Street address |
Anderson Stuart Building, room N452, University of Sydney
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE25076 |
Hypothalamic expression differences between hypertensive BPH/2J and normotensive BPN/3J mouse strains |
|
| Relations |
| Reanalyzed by |
GSM638230 |
