|
| Status |
Public on Jun 04, 2023 |
| Title |
D3.5ff-2-RNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
uterus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: uterus
Sex: female
pregnancy day: Pregnant day 3.5
genotype: TRIM28f/f
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Pgrcre/+TRIM28f/f and TRIM28f/f female mice were mated with wildtype males, and dissected at Pregnancy day 3.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol and purified using Qiagen Rneasy min kit
RNA libraries were prepared by Nextera DNA Flex kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Paired-end raw reads were filtered for quality score > 20.
Reads were mapped to the mouse genome (mm10) using Tophat version 2.0.4
Assembly: mm10
Supplementary files format and content: FPKM was calculated for each gene in each sample by using Cufflinks version 2.0.2.
|
| |
|
| Submission date |
Jun 03, 2022 |
| Last update date |
Jun 04, 2023 |
| Contact name |
Franco Demayo |
| E-mail(s) |
[email protected]
|
| Organization name |
NIEHS
|
| Street address |
111 TW Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE205478 |
TRIM28 modulates progesterone and estrogne signaling in endometrium [mouseD3.5-RNAseq-mm10] |
| GSE205481 |
TRIM28 modulates progesterone and estrogen signaling in endometrium |
|
| Relations |
| BioSample |
SAMN28861709 |
| SRA |
SRX15592651 |
