|
| Status |
Public on Dec 10, 2010 |
| Title |
NFAT5/TonEBP KO MEF cells, isotonic conditions |
| Sample type |
RNA |
| |
|
| Source name |
NFAT5/TonEBP knockout MEF cells, isotonic conditions
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic fibroblast (MEF) cells
genotype: NFAT5/TonEBP knockout
condition: isotonic
|
| Treatment protocol |
Non-synchronized cells were cultured for 20 hours in isotonic culture media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using a combined Trizol / Qiagen RNAeasy method
|
| Label |
Biotin
|
| Label protocol |
Total RNA was converted to first-strand cDNA using Superscript II reverse transcriptase primed by a poly(T) oligomer that incorporated the T7 promoter. Second-strand cDNA synthesis was followed by in vitro transcription for linear amplification of each transcript and incorporation of biotinylated CTP and UTP. The cRNA products were fragmented to 200 nucleotides or less, heated at 99degC for 5 min and hybridized for 16 h at 45degC to Affymetrix Mouse Gene-1_0-st-v1 microarrays
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| |
|
| Hybridization protocol |
The microarrays were washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain
|
| Scan protocol |
A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
|
| Data processing |
Affymetrix Microarray Suite 5.0 was used to quantitate expression levels for targeted genes; default values provided by Affymetrix were applied to all analysis parameters. Border pixels were removed, and the average intensity of pixels within the 75th percentile was computed for each probe. The average of the lowest 2% of probe intensities occurring in each of 16 microarray sectors was set as background and subtracted from all features in that sector. Probe pairs were scored positive or negative for detection of the targeted sequence by comparing signals from the perfect match and mismatch probe features. The number of probe pairs meeting the default discrimination threshold (tau = 0.015) was used to assign a call of absent, present or marginal for each assayed gene, and a p-value was calculated to reflect confidence in the detection call. A weighted mean of probe fluorescence (corrected for nonspecific signal by subtracting the mismatch probe value) was calculated using the One-step Tukey's Biweight Estimate. This Signal value, a relative measure of the expression level, was computed for each assayed gene. Global scaling was applied to allow comparison of gene Signals across multiple microarrays: after exclusion of the highest and lowest 2%, the average total chip Signal was calculated and used to determine what scaling factor was required to adjust the chip average to an arbitrary target of 150. All Signal values from one microarray were then multiplied by the appropriate scaling factor.
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| |
|
| Submission date |
Dec 02, 2010 |
| Last update date |
Dec 10, 2010 |
| Contact name |
Todd Lamitina |
| E-mail(s) |
[email protected]
|
| Phone |
215-898-3223
|
| Organization name |
University of Pennsylvania
|
| Department |
Physiology
|
| Street address |
3700 Hamilton Walk, A702
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE25816 |
Gene expression changes in NFAT5/TonEBP under isotonic and hypertonic conditions |
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