|
| Status |
Public on May 01, 2011 |
| Title |
female JAK2 WT liver sample 147 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse liver (8 weeks)
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed (C57Bl/6:129Sv) background
gender: female
tissue: Liver
sample number: 147
genotype/variation: WT
age: 8 weeks
|
| Growth protocol |
Animals were maintained on breeder chow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from mouse livers with TRIZOL (Invitrogen, Carlsbad CA) extraction, followed by purification with RNeasy Mini Column (QIAGEN, Valencia CA).
|
| Label |
biotin
|
| Label protocol |
Total RNA from 3 8-week old male and 3 female JAK2L and littermate control livers was prepared as above. RNA was analyzed using the Agilent 2100 BioAnalyzer. All samples were prepared using Affymetrix WT cDNA Synthesis and Amplification Kits for cDNA.synthesis, cRNA amplification and conversion to sense strand DNA products according to manufacturer's instructions. Samples were then fragmented and end labeled using Affymetrix WT Target Labeling and Control Reagents Kits according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Each sample was then hybridized to an Affymetrix Mouse Gene 1.0 ST array by standard procedures.
|
| Scan protocol |
Gene chips were scanned using the Affymetrix gene chip scanner 3000 7G
|
| Description |
gene expression data from female JAK2 WT mouse liver
FWT_147
|
| Data processing |
Data were analyzed using the Affymetrix Expression Console (algorithm-name = rma-gene-default) and then furhter analyzed using Microsoft Excel
|
| |
|
| Submission date |
Dec 20, 2010 |
| Last update date |
May 01, 2011 |
| Contact name |
Ethan J Weiss |
| E-mail(s) |
[email protected]
|
| Organization name |
UCSF
|
| Department |
CVRI
|
| Lab |
Weiss
|
| Street address |
555 Mission Bay Blvd South, Rm 382
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158-9001 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26188 |
Liver gene expression in animals with hepatocyte-specific deletion of JAK2 |
|
