|
| Status |
Public on Dec 23, 2010 |
| Title |
Drought treated leaves at panicle elongation stage, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Oryza DK151, leaf
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: leaf
genotype: DK151, a drought tolerant line from IR64
age: panicle elongation stage
|
| Treatment protocol |
Drought stress was processed by slowly draining the solution until leaf relative water content was 65%–75%.
|
| Growth protocol |
Rice plants were grown in PVC tubes (size: 75 cm×20 cm, each with a 20 cm hole from the bottom) with Turface and watered with 0.5×Yoshida.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using TRIzol reagents according to the instruction and then purified and concentrated using RNeasy MinElute Cleanup kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotin labeled cRNA was produced with 5 ug of total RNA as starting material for each sample reaction.
|
| |
|
| Hybridization protocol |
RNA from the three independent replicate samples were used for hybridization to the Affymetrix Genechips® Rice Genome Array chips, which contain probes to query 51,279 transcripts from two rice cultivars, including 48,564 japonica transcripts and 1,260 indica transcripts from Affymetrix (Santa Clara, CA).Hybridization to the arrays and quality control checks were carried out at the Company of CapitalBio Corporation, Beijing. (Affymetrix GeneChip Expression Analysis g of this fragmented target cRNA wasmTechnical Manual).
|
| Scan protocol |
Affy- Array Scanning
|
| Description |
DK151, a drought tolerant line from IR64
|
| Data processing |
The SAM (Significant Analysis of Microarray) software was applied to identify differentially regulated genes (DRGs) between samples using the two classes unpaired method based on the empirical criterion of more than two-fold change and significant q value (FDR adjusted P value) less than 0.05. A list of known or unknown genes was generated by performing a BLASTn search (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi) from the Affymetrix website
|
| |
|
| Submission date |
Dec 22, 2010 |
| Last update date |
Dec 23, 2010 |
| Contact name |
pan yajiao |
| E-mail(s) |
[email protected]
|
| Organization name |
The chinese academy of agriculture sciences
|
| Street address |
Zhongguancun South Street No.12
|
| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE26280 |
Genome-wide temporal-spatial gene expression profiling of drought responsiveness in rice |
|
