|
| Status |
Public on Oct 31, 2023 |
| Title |
HFD_4 |
| Sample type |
RNA |
| |
|
| Source name |
liver sample, HFD, 24 weeks
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
gender: male
strain: C57BL/6J
condition: HFD
duration: 24 weeks
|
| Treatment protocol |
Mice were maintained on a 12-h light-dark cycle with free access to water and 58% high fat diet (HFD) (Research Diets, D12331) for 24 weeks. All mice were fasted for 4 hours at the start of the light phase before being sacrificed by cervical dislocation for organ withdrawal.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from snap-frozen liver using the miRNeasy mini kit (Qiagen, Germany) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
microRNA expression from pre-diabetic murine liver
|
| Data processing |
Data analysis was performed with the Transcriptome Analysis Console software (version 4.0.2.5) with the analysis type 'Expression (Gene)'. As summarization method 'RMA+DABG (Mouse Only)' was used for signal normalization. A probe set was considered expressed if at least 50% of the detection above background (DABG) values were below the DABG threshold.
|
| |
|
| Submission date |
Aug 16, 2022 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Henriette Kirchner |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Lübeck
|
| Department |
Institute for Human Genetics
|
| Lab |
Epigenetics and Metabolism
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| ZIP/Postal code |
23562 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE211367 |
microRNA expression data from liver samples of mice fed with a high fat diet for 24 weeks |
|
