|
| Status |
Public on Jan 06, 2012 |
| Title |
oocyte_DirectLysis_rep3 |
| Sample type |
other |
| |
|
| Source name |
single oocyte, crude lysate
|
| Organism |
Mus musculus |
| Characteristics |
tissue: oocyte
strain: ICR
sample prep: NuGEN direct lysis
|
| Growth protocol |
two-month-old female mice (ICR strain) were intraperitoneally injected with 10IU PMSG, followed 46 hours later with 10IU hCG. Non-mated female mice were used for MII oocyte collection and mated females were used for collection of fertilized single-cell embryos. 1 to 3 cells were collected from each of 3 mice per condition.
|
| Extracted molecule |
other |
| Extraction protocol |
Qiagen RNeasyMini, Arcturus PicoPure, or NuGEN direct lysis per manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
NuGEN WT Ovation One-Direct amplification followed by NuGEN Encore Biotin module
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45 C on Mouse Gene 1.0ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000 7G.
|
| Description |
probe group file: MoGene-1_0-st-v1.r4.pgf
meta-probeset file: MoGene-1_0-st-v1.r4.mps
|
| Data processing |
Affymetrix GCOS, Affymetrix Expression Console, gc-RMA
|
| |
|
| Submission date |
Jan 06, 2011 |
| Last update date |
Jan 06, 2012 |
| Contact name |
Don Baldwin |
| E-mail(s) |
[email protected]
|
| Phone |
215-898-3675
|
| Fax |
215-898-4227
|
| URL |
http://www.med.upenn.edu/microarr
|
| Organization name |
University of Pennsylvania
|
| Department |
Pathology and Lab Medicine
|
| Lab |
Penn Microarray Facility
|
| Street address |
277 John Morgan, 3620 Hamilton Walk
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26486 |
Simple and efficient transcriptome profiling from a single embryo |
|
