|
| Status |
Public on Apr 06, 2011 |
| Title |
wild-type 3 |
| Sample type |
RNA |
| |
|
| Source name |
Flow-sorted CD4/CD8 DP thymocytes
|
| Organism |
Mus musculus |
| Characteristics |
tissue: thymus
strain: C57 Bl/6
genotype: wild-type
treatment: none
|
| Growth protocol |
Mice were all between 3 and 6 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Qiagen Rneasy Mini kit, with on-column DNAse digestion
|
| Label |
biotin
|
| Label protocol |
Labeling was in accordance with Affymetrix WT labeling kit
|
| |
|
| Hybridization protocol |
In accordance with manufacturer's reccomendation for Gene 1.0ST arrays
|
| Scan protocol |
Affymetrix GCS3000 scanner and the GCOS 1.4 data acquisition software
|
| Data processing |
.cel files were normalized using Exppression Console RMA
Matrix values represent log2 normalized scalar expression values for the indicated Affy probe sets
|
| |
|
| Submission date |
Jan 06, 2011 |
| Last update date |
Apr 06, 2011 |
| Contact name |
Eric Verdin |
| E-mail(s) |
[email protected]
|
| Phone |
(415) 209-2250
|
| Organization name |
Buck Institute for Research on Aging
|
| Department |
NA
|
| Lab |
Verdin
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26488 |
Differential Gene Expression in HDAC7-Deficient and Transgenic Thymocytes |
|
