|
| Status |
Public on Jan 20, 2011 |
| Title |
Tregs purified from MHC II deficient recipient, Biological Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Tregs purified from MHC II deficient recipient
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 6-8 weeks of age
origin tissue: Peripheral lymph nodes and spleen
cell type: CD4 Tregs lacking TCR
|
| Treatment protocol |
CD4+ TCRβ+ CD25+ cells were isolated by magnetic bead enrichment followed by fluorescence activated cell sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Rneasy Mini kit (QIAGEN) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip murine Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Description |
Gene expression data from CD4 Tregs lacking TCR / MHC class II interactions for 5 days (CD4+ TCRβ+ CD25+ cells purified from Total T cells transferred in CD3e / MHC II double deficient mice)
|
| Data processing |
The data were analizing with affy R package (RMA) and statistical analysis were then performed with MeV software (TIGR, Rockville MD)
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Jan 21, 2011 |
| Contact name |
Florent Dumont |
| E-mail(s) |
[email protected]
|
| Organization name |
Université Paris-Saclay
|
| Street address |
17 avenue des sciences
|
| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE26714 |
Expression data from Tregs adoptively transferred in MHC II competent or deficient recipients |
| GSE27153 |
Expression data from self-deprived regulatory CD4 T cells |
|
