|
| Status |
Public on Jan 27, 2011 |
| Title |
P25AN13282 8oxoG, technical rep.-extract 1 |
| Sample type |
RNA |
| |
|
| Source name |
8-oxoguanine treated MRC5 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human fibroblast
cell line: MRC5
treatment: 8-oxoguanine
time: 12 h
|
| Treatment protocol |
Cells were treated with 10µM 8-oxoguanine or mock treated with solvent for 6 hours.
|
| Growth protocol |
MRC5, human diploid fibroblasts were maintained at 37 oC, in 5% CO2, in EMEM medium, supplemented with 10% FBS, glutamine, penicillin, and streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Ambion's RNAqueous Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized to Affymetrix HG-U133A 2.0 Arrays in an Affymetrix Hybridization Oven-640 according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data 12 hours after 8-oxoguanine exposure
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 24, 2011 |
| Last update date |
Jan 27, 2011 |
| Contact name |
Istvan Boldogh |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Texas Medical Branch at Galveston
|
| Street address |
301 University Blvd.
|
| City |
Galveston |
| State/province |
TX |
| ZIP/Postal code |
77555 |
| Country |
USA |
| |
|
| Platform ID |
GPL201 |
| Series (1) |
| GSE26813 |
Expression data from human fibroblasts 6 and 12 hours after 8-oxoguanine treatment |
|
