|
| Status |
Public on Jul 26, 2011 |
| Title |
Men1flf, RIP-Cre pancreatic islet repeat 2 |
| Sample type |
RNA |
| |
|
| Source name |
pancreatic islets
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Men1flf, RIP-Cre
|
| Treatment protocol |
Mice were not treated.
|
| Growth protocol |
All mice were maintained at the research animal facility of the Dana-Farber Cancer Institute.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pancreatic islets were isolated from mice . Total RNA was harvested using RNeasy mini kit with on-column DNase digestion (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Fragmented single-stranded DNA was labeled by TdT using the GeneChip® WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Fragmented and labeled DNA target was hybridizated to Gene 1.0 ST arrays using the GeneChip® Hybridization, Wash and Stain Kit.
|
| Scan protocol |
The probe array was scanned using an Affymetrix® GeneChip® Scanner 3000.
|
| Description |
Pancreatic islets from 2 months old mice with the Men1 Gene knocked out
|
| Data processing |
Data were processed with dChip. Cel files were quantile normalized using MM2010052537.CEL as a base line. Signal values were calculated with the average modelling method using the 5th percentile of regions for background subtraction.
|
| |
|
| Submission date |
Jan 31, 2011 |
| Last update date |
Jul 26, 2011 |
| Contact name |
Andrew Cherniack |
| E-mail(s) |
[email protected]
|
| Organization name |
Broad Institute
|
| Street address |
301
|
| City |
Binney St |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26978 |
Expression data from pancreatic islets from Men1flf RIP-Cre mice, Rbp2flf RIP-Cre mice, Men1flf Rbp2flf RIP-Cre mice and matched control. |
|
