|
| Status |
Public on Feb 04, 2011 |
| Title |
SSC_Aged in vitro_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Young donor SSCs cultured for 14-18 months
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Spermatogonial Stem Cell
age: 8d donor, cultured 14-18 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Mouse Gene 1.0ST Arrays. GeneChips were washed and stained.
|
| Scan protocol |
GeneChips were scanned using a confocal scanner after excitation at 570 nm.
|
| Description |
Gene expression data from 8d donor SSCs, cultured 14-18 months
|
| Data processing |
The data were analyzed with Partek Genomics Suite v 6.4 where RMA was applied to normalize and summarize data to yield log2-transformed signal intensities for each probe set.
|
| |
|
| Submission date |
Feb 03, 2011 |
| Last update date |
Feb 04, 2011 |
| Contact name |
Zhiyv Niu |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pennsylvania
|
| Department |
Animal Biology
|
| Lab |
Reproductive Biology
|
| Street address |
One baylor plaza, 506E
|
| City |
houston |
| State/province |
tx |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27043 |
Expression data from aged spermatogonial stem cells |
|
