|
| Status |
Public on Dec 08, 2011 |
| Title |
Clock positive bulge cells 2 |
| Sample type |
RNA |
| |
|
| Source name |
Venus GFP positive Bulge cells (alpha6bright/CD34+/Venushigh population) isolated from the dorsal skin of P18-19 mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Dorsal skin bulge epidermal stem cells
cell phenotype: Venus GFP positive Bulge cells (alpha6bright/CD34+/Venushigh population)
|
| Treatment protocol |
There was no treatment prior to the purification of the cells
|
| Growth protocol |
Mice were housed under standard SPF conditions and according to the Ethical Committee for Animal Experimentation of the Government of Catalonia.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from FACS-sorted cells in Trizol by chloroform extraction, followed by the RNA clean-up protocol of RNeasy Micro or Mini Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
The indicated starting amount of total RNA was amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and subsequently labeled using GeneChip Mapping 10K Xba Assay Kit (Affymetrix; catalog # 900441), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Human Gene 1.0ST arrays were hybridized with 10 ug of labeled, amplified dsDNA, washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol FS450_007)
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 7G
|
| Description |
Gene expression data from purified clock positive bulge stem cells
|
| Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003)
|
| |
|
| Submission date |
Feb 04, 2011 |
| Last update date |
Dec 08, 2011 |
| Contact name |
Salvador Aznar Benitah |
| E-mail(s) |
[email protected]
|
| Phone |
+34 934034021
|
| Organization name |
Institute for Research in Biomedicine (IRB, Barcelona)
|
| Department |
Oncology
|
| Lab |
Stem Cells and Cancer
|
| Street address |
Baldiri Reixac 10
|
| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27079 |
Expression data from epidermal stem cells isolated from dorsal skin of P19 Per1-Venus mice and Bmal1 epidermal knockout mice |
|
