|
| Status |
Public on Mar 31, 2011 |
| Title |
ES undifferentiated-amplified rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Day 0 undifferentiated ES cells; sorted Sox2bright
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ES cells
strain: C57BL/6x129SvJae
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from three biological replicates corresponding to different cell and tissue types using miRNeasy Mini Kit (Qiagen,217004) according to the manufacturer’s protocol, and the WT-OvationTM Pico RNA amplification system (Nugen) was used to amplify the RNA
|
| Label |
biotin
|
| Label protocol |
EncoreT Biotin Module (Nugen)
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® Mouse Gene 1.0 ST arrays
|
| Scan protocol |
affy scanner
|
| Description |
Gene expression data from ES cells, iPS cells and Mouse Embryos
|
| Data processing |
data in this table were processed with RMA method at different batches, ES/iPS experiment consisting of samples GSM668576 to GSM668593 is the first batch, and ES/Embryos experiment consisting of samples GSM668564 to GSM668575 is the second batch.
|
| |
|
| Submission date |
Feb 04, 2011 |
| Last update date |
Dec 16, 2011 |
| Contact name |
steve shen |
| Organization name |
New York University
|
| Department |
Biochemistry
|
| Street address |
545 First Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27087 |
Mouse embryonic and induced pluripotent stem cells can form definitive endoderm despite differences in imprinted genes |
|
