|
| Status |
Public on Dec 30, 2011 |
| Title |
ES2, G4, M1, V4h |
| Sample type |
RNA |
| |
|
| Source name |
mouse vagina, 4h post treatment
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
gender: female
age: 7-9
treatment: α-GalCer
|
| Treatment protocol |
Mice were given Depo Provera (long lasting progestin) subconateously prior to being administered intravaginally with either adjuvant (CpG ODN or α-GalCer) or their respective control buffers, PBS and PBS/Tween 0.5%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Vaginas (cut below cervix) were surgically removed at different time-points following treatment and RNA was extracted by Rneasy according to manufacturer's instructions.
|
| Label |
Biotin Allonamide Triphosphate
|
| Label protocol |
Labeling reaction with Biotin Allonamide Triphosphateat was performed at 37 degrees
|
| |
|
| Hybridization protocol |
200 ng of sample was hybridized according to Affymetrix´s recommended protocoll (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay, 701880 Rev. 5).
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instructions (Affymetrix) by a Gene chip Fluidics station FS-400 and gene chip scanner 3000 7G
|
| Description |
Gr 4, mouse vagina, 4h
|
| Data processing |
Probe logarithmic Intensity Error (PLIER) with quantile normalization
|
| |
|
| Submission date |
Feb 08, 2011 |
| Last update date |
Dec 30, 2011 |
| Contact name |
Ali Mohaghegh Harandi |
| E-mail(s) |
[email protected]
|
| Phone |
+46(0)317866229
|
| Organization name |
University of Gothenburg
|
| Department |
Microbiology and Immunology
|
| Street address |
Medicinaregatan 7A
|
| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27149 |
Expression data from murine vaginal samples following adjuvant treatment |
|
