|
| Status |
Public on May 02, 2012 |
| Title |
TNd6_wt3 |
| Sample type |
RNA |
| |
|
| Source name |
TN day 6 of neurons
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129SvEv
cell type: TN day 6 of neurons
genotype/variation: wild type
|
| Treatment protocol |
Cells were treated with either DMSO or 50 μM ICRF-193 of the drug every 24 hours with fresh from TN day 2 to TN day 5.
|
| Growth protocol |
For Stem Cell differentiation transcritome analysis, wild type and Top2β knockout cells were differentiated as previously described (Bibel et al. 2004; Plachta et al. 2004; Bibel et al. 2007)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
RNA was processed according to the GeneChip Whole Transcript (WT) Double-Stranded Target Assay Manual from Affymetrix
|
| |
|
| Hybridization protocol |
hybridization was performed according to standard Affymetrix procedure
|
| Scan protocol |
Scanning was performed with Affymetrix GCC Scan Control v. 3.0.0.1214
|
| Data processing |
probesets were summarized and probeset-level values normalized with rma(target=core) function from R/Bioconductor package oligo (version 1.14.0)
|
| |
|
| Submission date |
Feb 11, 2011 |
| Last update date |
May 02, 2012 |
| Contact name |
Dirk Schuebeler |
| Organization name |
Friedrich Miescher Institute for Biomedical Research
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE27245 |
Expression data from Top2β KO cells as well ICRF-193 treatment of in vitro derived neurons and cortical glutamatergic neurons |
| GSE27247 |
Topoisomerase IIbeta occupies H3K4 methylated sites and regulates neuronal survival via repression of the neurotrophin receptor p75 |
|
