|
| Status |
Public on Jan 01, 2012 |
| Title |
Oma1_WT |
| Sample type |
RNA |
| |
|
| Source name |
White adipose tissue derived from Oma1 WT mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: white adipose tissue
genotype: Control mouse
age: 24 weeks old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Affymetrix Mouse Gene 1.0 ST Array according to the protocols recommended by the manufacturer (Affymetrix, Santa Clara, CA, USA).
|
| Scan protocol |
Washing and scanning were performed using Fluidics Station 400 and GeneChip Scanner (Affymetrix Inc.).
|
| Description |
Gene expression data from white adipose tissue derived from mouse maintaing 20 weeks with high-fat diet.
|
| Data processing |
After scanning, rawdata (Affymetrix Cel files) were processed with RMAExpress (http://RMAExpress.bmbolstad.com), using default settings
|
| |
|
| Submission date |
Feb 25, 2011 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Jose M.P. Freije |
| E-mail(s) |
[email protected]
|
| Phone |
34-985106281
|
| Organization name |
Universidad de Oviedo
|
| Department |
Bioquimica y Biologia Molecular
|
| Lab |
IUOPA
|
| Street address |
C/ Fernando Bongera s/n
|
| City |
Oviedo |
| State/province |
Asturias |
| ZIP/Postal code |
33006 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27525 |
Expression data from diet-induced obesity Oma1-deficient mice. |
|
