|
| Status |
Public on Mar 22, 2011 |
| Title |
1aDF macrophage rep3 |
| Sample type |
RNA |
| |
|
| Source name |
1aDF macrophages
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: SREBP-1a deficinet
macrophages: Bone marrow drived macrophage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on mouse gene 1.0 ST array GeneChips Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from embryos in slow phase of cellularisation.
|
| Data processing |
The data were analyzed with GeneChIP operating software using Affymetrix and GeneSpring software (Agilent technologies). The data were further analyzed by the Cyber-T statistical analysis program (http://cybert.microarray.ics.uci.edu).
|
| |
|
| Submission date |
Mar 18, 2011 |
| Last update date |
Mar 22, 2011 |
| Contact name |
Seung-Soon Im |
| E-mail(s) |
[email protected]
|
| Phone |
407-745-2000
|
| Organization name |
Sanford-Burnham Medical Research Institute
|
| Lab |
Dr. Osborne
|
| Street address |
6400 Sanger Rd.
|
| City |
Orlando |
| State/province |
FL |
| ZIP/Postal code |
32827 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE28037 |
Gene expression data from WT and SREBP-1a deficient macrophages |
|
