|
| Status |
Public on Mar 23, 2011 |
| Title |
CH biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
cholesterol fed
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
strain: C57BL/6
age: 6-week old
gender: male
|
| Treatment protocol |
All animal experiments were performed in accordance with accepted standards of animal welfare and with permission of the University of California, Irvine IACUC (protocol 97-1545). One group was maintained on a chow diet and the other two groups were fed either normal chow supplemented with a mixture of lovastatin (100 mg lovastatin [2.5 tablet equivalents]/100 g chow, w/w; Mylan) and ezetimibe (from MERCK/Schering-Plough Pharmaceuticals; 21 mg ezetimibe [2.1 tablet equivalents]/100 g chow, w/w) (LE) or powdered cholesterol (1%, w/w) (Ch).
|
| Growth protocol |
Six-week-old male C57BL/6 mice were obtained from Taconic and maintained on chow diet (2020X, Harlan Teklad Global ) for one week with a 12 hr light 12 hr dark cycle for acclimatization. 6 mice of two groups are subjected as Materials and Methods
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
See manufacturer's website (Affymetrix)
|
| |
|
| Hybridization protocol |
The Mouse Gene 1.0 OST (Affymetrix) by hybridizing RNA from Cholesterol and L/E in triplicate
|
| Scan protocol |
See manufacturer's website (Affymetrix)
|
| Description |
Gene Expression from mouse liver
|
| Data processing |
The data were analyzed with the Mouse Gene 1.0 OST using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 25. Differential expression was then assessed using the cyberT analysis program
|
| |
|
| Submission date |
Mar 22, 2011 |
| Last update date |
Mar 23, 2011 |
| Contact name |
Jacob Biesinger |
| E-mail(s) |
[email protected]
|
| Phone |
949 231 7587
|
| Organization name |
University of California, Irvine
|
| Department |
Computer Science
|
| Lab |
Xiaohui Xie
|
| Street address |
4058 Donald Bren Hall, University of California Irvine
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE28083 |
Expression data from CH/LE Mice |
| GSE28084 |
Genome-wide Localization of SREBP-2 in Hepatic Chromatin Predicts a Novel Role in Autophagy |
|
