|
| Status |
Public on Aug 23, 2011 |
| Title |
HFD3-VAT |
| Sample type |
RNA |
| |
|
| Source name |
Epididymal fat, high fat diet
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: epididymal adipose tissue (VAT)
treatment: high fat diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA minilipid kit (Qiagen) using the manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Ambion WT Expression Kit, Affymetrix WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol (GeneChip® Expression Analysis Technical Manual).
|
| Scan protocol |
Affymetrix® GeneChip® Scanner 3000 with GCOS software according to the manufacturer's protocol (GeneChip® Expression Analysis Technical Manual).
|
| Description |
Whole tissue isolate.
Mice fed for 13 wks with 45% kcal HFD.
|
| Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
| |
|
| Submission date |
Apr 06, 2011 |
| Last update date |
Aug 23, 2011 |
| Contact name |
Timothy P Fitzgibbons |
| E-mail(s) |
[email protected]
|
| Phone |
508-856-6573
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular Medicine
|
| Street address |
55 Lake Avenue North
|
| City |
Worcester |
| ZIP/Postal code |
01655 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE28440 |
Gene expression from mouse white, brown, and perivascular adipose tissue |
|
