|
| Status |
Public on Sep 04, 2011 |
| Title |
testis gene expression data from Akt1 heterozygous, PTU-exposed mice at postnatal day 15, sample 3 |
| Sample type |
RNA |
| |
|
| Source name |
PTU-exposed testes at postnatal day 15
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Akt1 heterozygous
treatment: PTU-exposed
developmental stage: postnatal day 15
sample number: sample 3
gender: male
strain: C57BL6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was performed according to the TriReagent manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Equal amounts of purified cDNA per sample were used as the template for subsequent in vitro transcription reactions for complementary RNA (cRNA) amplification and biotin labeling using an Affymetrix RNA Transcript Labeling Kit. cRNA was purified and fragmented according to the protocol provided with the GeneChip Sample Cleanup Module (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
All GeneChip microarrays (Mouse Genome MoGene-1_1-st-v1 Array) were hybridized, washed, stained, and scanned using the Complete Gene Chip Instrument System according to the Affymetrix Technical Manual.
|
| Scan protocol |
scanned using the Complete Gene Chip Instrument System according to the Affymetrix Technical Manual.
|
| Data processing |
RMA in R Bioconductor
|
| |
|
| Submission date |
Apr 15, 2011 |
| Last update date |
Sep 04, 2011 |
| Contact name |
Mary Hixon |
| Organization name |
Brown University
|
| Street address |
70 Ship St
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE28664 |
Akt1 is critical in Maintaining the Blood-Testis Barrier Following Exposure to the Neonatal Goitrogen, 6-N-Propylthiouracil (PTU) |
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