|
| Status |
Public on Jul 26, 2011 |
| Title |
9949 transgenic animal |
| Sample type |
RNA |
| |
|
| Source name |
transgenic mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: primary liver tissue
background strain: C57BL/6 x 129/SV
|
| Treatment protocol |
Transgenic and control mice were treated with doxycycline (1mg/d i.p.) three times (0, 24 and 48h) and liver samples were collected after 52h. In addition, primary hepatocytes isolated from transgenic and control animals were cultured with doxycycline for 3 days (1µg/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted from cells with RNAeasy kit including DNAse digestion (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). For the Affymetrix array, 300ng of total RNA were used. Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix)
|
| Scan protocol |
Arrays were scanned in a GeneChip scanner 3000 (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software; core-exon analysis
|
| Data processing |
Normalization and statistical data analyses were performed by an RMA algorithm performed with GeneSpring GX 10 software (Agilent)
|
| |
|
| Submission date |
Apr 18, 2011 |
| Last update date |
Jul 26, 2011 |
| Contact name |
Peter Kuckenberg |
| URL |
http://www.meb.uni-bonn.de/devpatho/
|
| Organization name |
University Medical School Bonn
|
| Department |
Institut of Pathology
|
| Lab |
Prof. Schorle
|
| Street address |
Sigmund Freud Str. 25
|
| City |
Bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE28692 |
Transgenic overexpression of Tcfap2c/AP-2gamma results in liver steatosis |
|
