|
| Status |
Public on Apr 29, 2011 |
| Title |
polyploidy sample3 |
| Sample type |
RNA |
| |
|
| Source name |
pregnancy Day 7 decidual cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD-1
tissue: uterus
cell type: polyploid
Sex: female
|
| Treatment protocol |
Deciduomal tissues were separated from the muscle and minced by scalpel blade, and then placed in HBSS containing 0.25 % collagenase, 0.1 % DNase I, and 0.1 % hyaluronidase for 45 min. at 37o C. The enzyme digested tissues were then serially passed through 18, 22, and 25 gauge needles to make a single cell suspension.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted from polyploid and non-polyploid decidual cell populations using RNeasy Mini Kit according to the manufacturer’s instructions (QIAGEN, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNAs were generated using the Affymetrix Whole Transcript Sense Target Labeling Assay (Affymetrix)
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNAs were hybridized to GeneChip® mouse gene 1.0 ST array (Affymetrix Inc.).
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G used to scan and quantitate the gene chips using default scan settings
|
| Description |
Das-P3
|
| Data processing |
Differentially expressed genes were selected based on the GeneSpring GX10 program, with a threshold of unpaired t-test (P-value < 0.05), false discovery rate (FDR) < 5%, and a fold change cut off = 2.0.
|
| |
|
| Submission date |
Apr 28, 2011 |
| Last update date |
Apr 29, 2011 |
| Contact name |
xinghong ma |
| E-mail(s) |
[email protected]
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE28917 |
Development of decidual cell polyploidy and bi-nucleation necessitates mitochondrial activity |
|
