|
| Status |
Public on Apr 23, 2012 |
| Title |
Mock Treated - 3 days - repeat 3 - mAdbID:115868 |
| Sample type |
RNA |
| |
|
| Source name |
Mock cell Treated - 3 days
|
| Organism |
Mus musculus |
| Characteristics |
tissue: subcutaneous B16 melanoma tumor
disease state: melanoma
other: Subcutaneous B16 tumors were excised from mice 3 days following treatment with 1 X 10(5) Mock pmel-1 CD8+ cells and single cell suspensions were resuspended into RLT buffer (Qiagen) and frozen at -80° F until analysis.
|
| Treatment protocol |
Treatment type: adoptive transfer therapy
Treatment dose: 1 x 10(5) Mock cells
Treatment time: 3 days
In-vivo treatment: Six to twelve week old mice were injected subcutaneously with 5 x 10(5) B16 melanoma cells. Ten to fourteen days later, they were irradiated with 5 Gy total body irradiation (TBI) and given 1 x 10(5) un-transduced pmel-1 CD8+ T cells (Mock cells).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sample Extraction Protocol
Other: Total RNA was isolated using the RNeasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Affymetrix Labeling Protocol
Other: Fragmented single stranded sense DNA were terminal labeled according to the manufacturers' instructions.
|
| |
|
| Hybridization protocol |
Affymetrix Hybridization Protocol
Other: Fragmented single stranded sense DNA were hybridized to Mouse GeneChip 1.0 ST array (Affymetrix) according to the manufacturers' instructions.
|
| Scan protocol |
Affymetrix Scanning Protocol
Other: Arrays were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
mAdb experiment ID: 115868
|
| Data processing |
Affymetrix RMA Data Processing
Calculation Method: Expression summary values were calculated using the Bioconductor implemented version of RMA (Robust Multichip Average).
|
| |
|
| Submission date |
May 09, 2011 |
| Last update date |
Apr 23, 2012 |
| Contact name |
Francesco Maria Marincola |
| E-mail(s) |
[email protected]
|
| Phone |
301-793-8210
|
| Organization name |
Sidra Medical and Research Center
|
| Street address |
Al Nasr Tower, AL Corniche Street, PO Box 26999
|
| City |
Doha |
| ZIP/Postal code |
PO Box 26999 |
| Country |
Qatar |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE29164 |
IL-12 induces myeloid cells in situ to cross-present antigen |
|
